Some vectors also include elements that allow them to be maintained in another organism in addition to E. coli, and these vectors are called shuttle vector. Cloning site. Selectable marker. Reporter gene. Elements for expression. Plasmid. Bacteriophage. Cosmid. Bacterial artificial chromosome. Features of a cloning vector - Cloning site - Selectable marker - Bacteriophage. The Major Types of Cloning Vectors. Plasmid. Circular extrachromosomal DNA that autonomously replicates inside the bacterial cell. Phage. Linear DNA molecules derived of bacteriophage lambda. Cosmids. Bacterial Artificial Chromosomes. Types of cloning vectors used in gene cloning: Plasmid (PBR): Bacterial artificial chromosome (BAC): Yeast artificial chromosome (YAC): figure: pYAC3. Example: pYAC3. Λ-Bacteriophase: figure: lambda bacteriophase. Expression vector: figure: expression vector pSG5. Cosmid: figure: Cosmid. Human artificial chromosome.
The following points highlight the six main types of cloning vectors. The types are: 1. Plasmid Vectors 2. Bacteriophages as Vectors 3. Cosmids as Vectors 4. Gene cloning is a major breakthrough, the important part of which is cloning vectors. The various uses of cloning remain redundant if a suitable. Different types of cloning vectors are used for different types of cloning experiments. • The vector is chosen according to the size and type of DNA to be cloned.
Objectives: After the end of the presentation we'll know - What is cloning vector? Why cloning vector? History Features of a cloning vector Types. Types of Vectors. Cloning vectors provide a backbone for the DNA insert to be reproduced and propagated in bacteria; however, these vectors are only useful for. Types of cloning vectors. • plasmids. • filamentous phages. • cosmids. • bacterial artificial chromosomes (BACs). • yeast artificial chromosomes (YACs). • lambda.